Khisal Alvi Ph.D. 10366 Reserve Dr. # 101 San Diego, CA 92127 Office (858) 426−2552 Home (858) 676−9754 khisal@hotmail.com
PROFESSIONAL SUMMARY
Over 14 years of industrial (Pharmaceutical/Biotech) experience in Natural Products, Analytical chemistry and Bioprocess Development. Experienced in new drug discovery chemistry, QC and bioprocess development and modern experimental techniques; Possesses excellent oral and written communication skills, interpersonal skills and management skills.
Thorough knowledge of natural product chemistry, Analytical methodologies and Bioprocess development.
Extensive skills in chemical and instrumental methods of analysis, exceptional expertise in method development, optimization and validation
Extensive experience in isolation and structure−elucidation of small molecules.
Extensive experience in chromatographic and spectroscopic techniques (e.g. HPLC, CCC, FPLC, NMR, LC−NMR, MS, LC−MS etc.)
Hands−on expertise in HPLC, NMR, LC−NMR, MS, LC−MS, UF/DF, AKTA Crossflow, SDS−PAGE, western blotting and ELISA
SELECTED ACCOMPLISHMENTS
Developed a new downstream process for purification of glycoprotein from plant tissue culture.
Developed a new and fast Ultra Performance Liquid Chromatography assay for analysis of paclitaxel and related compounds from plant tissue culture.
Introduced and implemented several processes and products in order to keep MDS Panlabs? and AMRI?s (Albany Molecular Research, Inc.) services competitive with other contract research organizations.
Built the Natural Products Chemistry group at Panlabs and managed several successful drug discovery and purification projects for Panlabs? clients.
Designed Panlabs Premium Extract Library (the industries first pre−fractionated extract library).
Introduced and implemented centrifugal partition chromatography technique for initial fractionation of crude extracts in drug discovery projects.
Developed high throughput fractionation and rapid de−replication protocols for streamlined natural products discovery process in order to reduce cycle time and cost.
Developed and optimized several procedures for the isolation of bioactive compounds in large scale (gram quantities).
Built a searchable UV spectrum library.
EXPERIENCE AND EMPLOYMENT
Vertex Pharmaceuticals, San Diego 2008−Present Analytical Development Oversee the NMR facility, contribute to the global structure elucidation program for Pharmaceutical Development and metabolite structure elucidation for research project. Phyton Biotech, East Windsor NJ 2006−2008 Manager, Process Development (2006−Present) Manage a group engage in development of purification process of recombinant proteins and small molecules such as paclitaxel from plant cell culture.
Responsible for development of scaled up purification processes from bench scale to pilot plant production scale.
Developed rapid, reliable assays to monitor progress of target protein purification using Bradford Protein estimation, SDS−PAGE, Western blot, ELISA, and SE HPLC.
Responsible for preparation of documents such as SOPs, batch records, and process development reports; presentation of development progress in project meetings; participation of technology transfer and scale−up process in the pilot plant and in the CMO manufacturing site.
Responsible for Identification of protein purification lab needs as company vision and company itself evolves, including assisting in budget proposals for personnel and equipment, planning and/or securing adequate facilities for lab operations, and recruiting/ hiring analytical lab personnel.
Phyton Biotech, East Windsor NJ 2005−2006 Manager, Analytical Chemistry (2005−2006)
Lead a group responsible for the development and validation of analytical methods (small molecules).
Manage analytical group responsible for supporting Phyton plant cell culture process, purification and recovery process, pilot plant, extractables and stability studies.
Responsible for organizing, advising, and activating task forces to investigate, to research and to further develop new and existing methods and procedures for analysis. Provide guidance for and expert advice to subordinates, where applicable.
Responsible for Identification of analytical lab needs as company vision and company itself evolves, including assisting in budget proposals for personnel and equipment, planning and/or securing adequate facilities for lab operations, and recruiting/ hiring analytical lab personnel.
Albany Molecular Research, Inc., Bothell Research Center, Bothell WA 1998−2005 SENIOR SCIENTIST III, Structure Analysis (2000 −2005) Appointed to lead Structure Analysis group (following acquisition of NCE by AMRI). Responsible for development and validation of analytical methods for the analysis of drug products and isolation and structure elucidation of bioactive molecules from natural products.
Led a team of 5 scientists in the effort to discover new drugs from natural products.
Developed and implemented de−replication procedure for quick identification of screening hits due to known compounds and/or known activity (resulting in reduction in cycle time and cost).
Developed several LC−NMR and LC−MS methodologies for the identification of impurities present in drug products.
Interacted with all groups at AMRI, Inc., to assist in development and implementation of new projects.
SENIOR SCIENTIST II, Separation & Analysis (1998−2000)
Supervised a group of 5 scientists responsible for isolation and characterization of bioactive compounds from natural products and synthetic mixtures.
Developed and implemented countercurrent chromatography (CCC), HPLC LC−NMR and LC−MS protocols for natural products and synthetics follow−up.
Operated and maintained LC−MS instruments.
Natural Products Chemistry, MDS PANLABS, Inc., Bothell WA. 1996−1998 SENIOR SCIENTIST (1996−1998) Managed a Natural Products Chemistry group, responsible for development of libraries from natural products extracts. Supervised 4 laboratory personnel.
Developed and optimized procedures and characterization of bioactive compounds from natural products.
Developed and optimized several procedures for the isolation of bioactive compounds in large scale (gram quantities).
Natural Products Chemistry, PANLABS, Inc., Bothell WA 1993−1996 RESEARCH SCIENTIST (1993−1996) Responsible for establishing the microbial fermentation based natural products chemistry program for high throughput screening. Isolation and identification of bioactive substances from natural products.
Developed and optimized procedures and characterization of bioactive compounds from natural products.
Operated and maintained LC−MS MS (PE SCIEX API III) and NMR instruments.
Department of Chemistry University of California, Santa Cruz, CA 1992
RESEARCH FELLOW Isolation and identification of novel bioactive substances from marine organisms.
University of California, Santa Cruz, CA 1989−1991 POSTDOCTORAL RESEARCH (Advisor: Prof. Phil Crews) Isolation and identification of novel bioactive substances from marine organisms.
University of Southampton, England (with Prof. Ian Steven) 1988−1989 Identification and total synthesis of fruit fly pheromone components.
MANAGERIAL & PROFESSIONAL TRAINING
"Design of Experiments−JMP Software" Successfully completed a Process Development course offered by GE Fast Trak training & Education program. Completed two leadership training courses; "Leadership Skills Training" by Grove Associates (12/2004) and "From the Laboratory to Leadership" by The Leadership Edge (03/2001). Operation and maintenance of LC−MS MS (PE SCIEX API III). Operation of NMR, LC−NMR instruments (GN 300, Bruker 500, 300, 250). Operation of FPLC (ÄKTA Explorer, Amersham Biosciences). Chromatographic Software (Water?s Millennium, 990, 991, Empower and Perkin−Elmer?s Chromafile3DTM plus PDA software). COMPUTER SKILLS PC−MOD (Molecular Mechanics), Excel, ChemText, ChemBase, Word, Dos, Windows ISIS, etc.
EDUCATION
Ph.D. H.E.J. Research Institute of Chemistry, University of Karachi, Pakistan, 1987. Organic Chemistry, advisor: Prof. Atta−ur−Rahman. Topic: Isolation and structure elucidation of natural products from terrestrial plants and total synthesis of pesticides. M.Sc. University of Karachi, Pakistan, 1983. Major: Organic Chemistry. B.Sc. Honors, University of Karachi, Pakistan, 1981. Major: Chemistry; Minor: Microbiology and Zoology.
HONORS
2000 − Recipient of UNDP (United Nation's Development Program) grant for undertaken a TOKTEN (Transfer of Knowledge Through Expatriate Nationals) assignment in Pakistan. 1989 − Recipient of Postdoctoral Scholarship from U.C. Cancer Research Coordinating Committee− UCSC. 1986 − Recipient of Pre−doctoral Scholarship from the University Grant Commission.
PROFESSIONAL ASSOCIATIONS
American Chemical Society American Society of Pharmacognosy Society for Industrial Microbiology New York Academy of Sciences The American Society of Mass Spectrometry
PRESENTATIONS
ORAL PRESENTATIONS "High Throughput Screening of Natural Products−−−−More Than A Number Game" Nair, B. Alvi, K., 1st International Conference: Advances in High Throughput Screening Technologies, Atlantic City, New Jersey, February 5−7,2001.
"Screening Natural Products: Bioassay−Directed Isolation of Active Components by Dual−Mode Centrifugal Partition Chromatography" Alvi, K. A., 1st International Conference on Countercurrent Chromatography, Uxbridge, Middlesex, UK, September 11−14,2000.
"Prefractionated Extract Library: A Strategy for Accelerating Natural Products Lead Discovery" Alvi, K. A. Baker, D., 41st Annual Meeting of the American Society of Pharmacognosy, Seattle, Washington, July 22−26,2000.
"Applications of Centrifugal Counter Current Chromatography" Alvi, K. A., Internal Workshop on Technology for Product Development from Medicinal Plants, HEJ Research Institute of Chemistry University of Karachi, Pakistan, January 21−27,2000.
"Strategy for Rapid Identification of Novel Therapeutical Leads from Natural Products", Alvi, K. A., Biologically Active Natural Products: Agrochemicals and Pharmaceuticals Symposium at 214th ACS National Meeting, Las Vegas, Nevada, September 7−11,1997.
"Dual Mode HSCCC−Photodiode Array Detector: An Approach for Rapid Fractionation of Microbial Extracts in High Through−put Screening" Alvi, K. A., Pu, H., Mocek, U., Peterson, J., SIM Annual Meeting, Research Triangle Park, North Carolina, August 4−9, 1996.
"Drugs from Sea" Alvi, K. A., HEJ Research Institute of Chemistry University of Karachi Pakistan, December, 15, 1995.
"Screening Natural Products. Bioassay−Directed Isolation of Active Compounds By Centrifugal Partition Chromatography" Alvi, K. A., Zymogenetics, Inc., November, 10, 1994.
"Sesquiterpenoid Quinones from the Dysidea Avara" Alvi, K. A., Crews, P., 204th American Chemical Society National Meeting, Washington DC, August 23−28, 1992.
"Protein Kinase C Inhibitor from Penaris sollas" Alvi, K. A., Crews, P., 33rd Annual Meeting American Society of Pharmacognosy, Colonial Williamsburg, Virginia, July 26−31,1992.
"Tyrosine Kinase Inhibitor Quinone Derivatives from Xestospongia carbonaria" Alvi, K.A., Crews, P., 203rd American Chemical Society National Meeting, San Francisco, Calif., April 5−10, 1992.
"2−Aminoimidazoles from the Nudibranch Notodoris Gardinei" Alvi, K. A., Crews, P., Pacific Conference on Chemistry and Spectroscopy, Foster City, Calif., October 20−25, 1992.
"What are the Implications of Isolating the Same Marine Natural Products from Organisms Within Different Phyla" Alvi, K. A., Crews, P., Pacific Conference on Chemistry and Spectroscopy, Foster City, Calif., October 20−25, 1992.
"Homoscalarane Sesterterpenes from L. frondosa" Alvi, K. A., Crews, P., Pacific Conference on Chemistry and Spectroscopy, San Francisco, Calif., October 20−25, 1990.
"Anthelminthic Polyfunctional Nitrogen Containing Terpenes from Marine Sponges" Alvi, K. A., Tenenbaum, L.,Crews, P., Pacific Conference on Chemistry and Spectroscopy, San Francisco, Calif., October 22−27, 1988.
POSTER PRESENTATIONS "Diverse natural product libraries and cutting−edge technology for the rapid identification and advancement of lead compounds" Alvi, K. A., Rabenstein, J. Mocek, U. and Baker, D., The Seventh International Conference on BiotechnologyMicrobial Products (BMP02 ), Honolulu, HI, October 27−30, 2002.
"Chemically diverse bio−active natural product compounds identified in both cell−based and mechanism−based assays" Baker, D. Mocek U., Alvi, K. A. and Rabenstein, J., The Seventh International Conference on BiotechnologyMicrobial Products (BMP02 ), Honolulu, HI, October 27−30, 2002.
"CD45 Protein Tyrosine Phosphatase Inhibitors From Microbial Extracts" Alvi, K. A., Nair, B., Rabenstein J., Baker, D., Sixth Conference on Biotechnology Microbial Products (BMP ?99) , May 16−19, 1999.
"Panlabs' Accelerated Drug Discovery Programs: From Natural Products Extracts to Lead Structures in Three Months" Laakso, J., Alvi, K. A., Hotovec, B., Pu, H., Mocek, U., 38th annual Meeting of the American Society of Pharmacognosy, Iowa City, July 26−30, 1997.
"Diverse Natural Product Libraries for use in High Throughput Screening Programs" Hotovec, B., Alvi, K. A., Laakso, J., Pu, H., Mocek, U.,38th annual Meeting of the American Society of Pharmacognosy, Iowa City, July 26−30, 1997.
"Phomacins: Three Novel Antitumor Cytochalasins Constituents of Phoma Sp." Alvi, K. A., Nair, B., Pu, H., Ursino, R., Gallo, C., Mocek, U.,Baker D. and Peterson J., 20th IUPAC Symposium on the Chemistry of Natural Products, Chicago, September 15−20, 1996.
"Rapid Identification of Elaiophylin and Geldanamycin in Streptomyces Fermentation Broth Using CPC Coupling with Photodiode Array Detector and LC−MS Methodology" Alvi, K. A., Peterson J., The Fourth International Conference on Biotechnology Microbial Products (BMP ?95), Oiso, Japan, April 23−26, 1995.
"Isolation and Identification of a New Cephem Compound from Penicillium chrysogenum Broth Expressing Deacetoxy−cephalosporin C Synthase Activity" Alvi, K. A., Reeves, C.D., Peterson J., Lein, J., The Fourth International Conference on Biotechnology Microbial Products (BMP ?95), Oiso, Japan, April 23−26, 1995.
"Identification of Potent Cytoproliferate Activity in Fermentation Broth Extract from Actinoplanes sp. on an Erythropoietin Responsive Cell Line: Initial Purification and Characterization of Activity" Ferrell, F., Wilkins, P., Alvi, K. A., Johnston, T., Tullai, J., Jollife, L. and Maechler, L., The Fourth International Conference on Biotechnology Microbial Products , Oiso, Japan, April 23−26, 1995.
"Rapid Identification of Elaiophylin and Geldanamycin in Streptomyces fermentation Broth Using CPC Coupling with Photodiode Array Detector and LC−MS Methodology" Alvi, K. A., Peterson J., International Conference on Microbial Secondary Metabolism , Interlaken, Switzerland, October, 1994.
"Isolation and Identification of a New Cephem Compound from Penicillium chrysogenum Broth Expressing Deacetoxy−cephalosporin C Synthase Activity" Alvi, K. A., Reeves, C.D., Peterson J., Lein, J., International Research Congress on Natural Products , Halifax, Nova Scotia, Canada, July 30−Aug. 4, 1994.
"Identification of an Active Metabolite from Streptomyces Violaceusniger in the Kainate Receptor Binding Assay" Henricksen, D. J., Leung, K. H Hosken, M. I., Baker, D. D., Alvi, K. A., Peterson J. R., Cochran, D. W., Hoffmann, B., R., Society of Industrial Microbiology, Boston, Massachusetts, July 30−Aug. 5, 1994.
"Structure of a New Taxane Diterpenoid from the Bark of Taxus Brevifolia" Alvi, K. A., Pu, H., Hotovec, B., Peterson, J., 34th Annual Meeting American Society of Pharmacognosy, San Diego California, July 18−22, 1993.
"Penazetidine−A, A Potent Selective Inhibitor of Protein Kinase C" Alvi, K. A., Crews, P., Marine Natural Products Gordon Research Conference, Ventura, California February 20−25 1992.
CHAPTERS
Alvi, K. A. "A Strategy for rapid identification of novel therapeutical leads from natural products" In Biologically Active Natural Products: Agrochemical and Pharmaceuticals, ed.Cutler, H.G., Cutler, S. J., CRC Press 1999, Chapter 14,185−195.
"Alvi, K. A., ?Discovery of antitumor and enzyme inhibitors from natural products" In Trends in Organic Chemistry, ed. Uma Ramchandran, Research Trends Press, 2001, 9, 69−96.
BOOK REVIEWS Scale−up and Optimization in Preparative Chromatography, Chromatographic Sciences, Vol. 88, ed. Rathore, A. Velayudhan, A. J. Nat. Prod., 2004, 67, 523. Cell Culture Technology for Pharmaceutical and Cell−Based Therapies. Ed. by S. S. Ozturk and W.−S. Hu, J. Nat. Prod., 2007, 70, 713.
LIST OF PUBLICATIONS
1. D. D. Baker, Alvi, K. A., Small−molecule natural products: new structures, new activity, Current Opinion in Biotechnology, 2004, 15, 576.
2. Alvi, K. A., Rabenstein, J. Auxarthrol A and auxarthrol B: two new tetrahydoanthraquinones from Auxarthron umbrinum, J. Ind. Microbiol. Biotechnol., 2004, 31, 11.
3. Alvi, K. A., Rabenstein, J., Woodard J., Baker, D. D., Bergthod, J. D., Lynch, J., Lieu, K. L., Braude, I. A. 14¢−Hydroxymytoxin B and 16−Hydroxyroridin E, Two New Cytotoxic Trichothecenes from Myrothecium roridum, J. Nat. Prod., 2002, 65, 742.
4. Alvi, K. A.,Screening Natural Products: Bioassay−Directed Isolation of Active Components by Dual−Mode CCC, J. Liq. Chrom. & Rel. Technol., 2001, 24, 1765.
5. Alvi, K. A., Baker, D., Stienecker, V., Hosken M., Nair, G. B., Identification of Inhibitors of Inducible Nitric Oxide Synthase from Microbial Extracts, J. Antibiotics, 2000, 53, 496.
6. Alvi, K. A., Nair, G. B., Rabenstein, J., Davis, G., Baker, D., CD45 Tyrosine Phosphatase Inhibitory Components from Aspergillus niger, J. Antibiotics, 2000, 53, 110.
7. Alvi, K. A. ?A Strategy for rapid identification of novel therapeutical leads from natural products? In Biologically Active Natural Products: Agrochemical and Pharmaceuticals, ed.Cutler, H.G., Cutler, S. J., CRC Press 1999, Chapter 14,185.
8. Alvi, K. A., Henry, P., Luche, M., Rice, A., App, H., McMahon, G., Dare, H. and Margolis, B., Asterriquinones Produced by Aspergillus candidus Inhibit Binding of the Grb−2 Adapter to Phosphorylated EGF Receptor Tyrosine Kinase, J. Antibiotics, 1999, 52, 215.
9. Alvi, K. A., Casey, A. and Nair, G. B., Pulchellalactam: A CD45 Protein Tyrosine Phosphatase Inhibitor from the Marine Fungus Corollospora pulchella, J. Antibiotics, 1998, 51, 515.
10. Alvi, K. A., Nair, G. B., Cathy G. and Dwight B., Screening of Microbial Extracts for Tyrosine Kinase Inhibitors, J. Antibiotics, 1997, 50, 264.
11. Alvi, K. A., Nair, G. B., Henry Pu, Rocky Ursino, Cathy Gallo and Ursula Mocek, Phomacins: Three Novel Antitumor Cytochalasins Constituents of Phoma Sp., JOC, 1997, 62, 2148.
12. Alvi, K. A., Peterson, J. and Hofmann, B., Rapid Identification of Elaiophylin and Geldanamycin in Streptomyces fermentation Broth Using CPC Coupling with Photodiode Array Detector and LC−MS Methodology, J. Industrial Microbiology, 1995, 15, 80.
13. Alvi, K. A., Reeves, C. D., Peterson, J. and Lein, J., Isolation and Identification of a New Cephem Compound from P. chrysogenum Strains Expressing Deacetoxycephalosporin Synthase Activity, J. Antibiotics, 1995, 48, 338.
14. Alvi, K.A., Jaspars, M. and Crews, P., Penazetidine−A, an Alkaloid Inhibitor of Protein Kinase C, Bioorganic & Medicinal Chemistry Letters, 1994, 4, 2447.
15. Alvi, K.A., Rodriguez, J., Lee, R.H., Slate, D.L. and Crews, P., Protein Tyrosin Kinase Inhibitory Properties of Plainar Polycyclic obtained from the Marine Sponge Xestospongia carbonieria and total synthesis, JOC, 1993, 58, 4871.
16. Alvi, K.A., Peters, B. M., Hunter, L. M. and Crews, P., 2−Aminoimidazoles and Their Zinc Complexes from Indo−Pacific Leucetta Sponge and Notodoris Nudibranchs, Tetrahedron, 1993, 49, 329.
17. Alvi, K.A., Crews, P., Lee, R.H., Slate, D.L., Moretti, R., Evaluation of New Sesquiterpenoid Quinones from two Dysidea Sponge Species as Inhibitors of Protein Tyrosine Kinase Activity from D. avara, JOC, 1992, 57, 6604.
18. Alvi, K.A., Crews, P., Homoscalarane Sesterterpenes from L. frondosa, J. Nat. Prod. 1992,55, 859.
19. Lee, R.H., Slate, D.L., Moretti, R., Alvi, K.A. and Crews P., Marine sponge Polykide, Inhibitors of Protein Tyrosine Kinase, Biochem. Biophys. Res. Commun.,1992,184, 765.
20. Todd, J.S., Alvi, K.A., Crews, P., The Isolation of a Monomeric Carboxylic Acid of Swinholide A from the Indo−Pacific Sponge Theonella swinhoei, Tetrahedron Lett., 1992, 33, 441.
21. Alvi, K.A., Crews, P., Novel Marine Sponge Alkaloids. 2. Structure and Total Synthesis of 2 aminoimidazoles from a, Notodorii Nudibranche, J. Nat.Prod. 1991, 54, 1509.
22. Alvi, K.A., Crews, P., Aalbersberg, B., Prasad,R.,Limonoids from the Fijian Medicinal Plant Dabi (Xylocarpus), Tetrahedron, 1991, 47, 8943.
23. Atta−ur−Rahman, Alvi, K.A., Abbas, S.A., Sultana, T., Choudhary, M.I., and Clardy, J., A Diterpenoid Lactone from Aplysia juliana, J. Nat. Prod., 1991, 54, 886.
24. Atta−ur−Rahman, Abbas, S.A., Nighat, F., Ahmed, A., Choudhary, M.I., Alvi, K.A., Habib−ur−Rehman, De Silva, K.T.D. and Arambewela, L.S.R., Chemical Constituents of Alstonia macrophylla, J. Nat. Prod., 1991, 54, 750.
25. Alvi, K.A., Tenenbaum, L.,Crews, P., Anthelminthic Polyfunctional Nitrogen Containing Terpenes from Marine Sponges, J. Nat. Prod., 1991, 54, 71.
26. Atta−ur−Rahman, Abbas, S.A., Alvi, K.A., and Clardy, J., Zoanthaminone, A New Alkaloid from a Marine Zoanthids, Tetrahedron Lett., 1989, 33, 6825.
27. Atta−ur−Rahman, Ahmad, V. U., Bano, S., Abbas, S.A., Alvi, K.A., Shaiq, M., Helen, L. and Clardy, J., Pinnatazane Cyclic Ether from Laurencia pinnatifide, Phytochemistry, 1988, 27, 3879.
28. Gamalath, C. B., Gunatilaka, A.A. L, Alvi, K.A., and Atta−ur−Rahman, Balasubramaniam, S., Studies on Medicinal and Related Plants of Sri Lanka. Part 17. Curcurbitacins of Colocybthis vulgaris, Phytochemistry, 1988, 27, 3225.
29. Atta−ur−Rahman, and Alvi, K.A., Indole Alkaloids Alstonamine and Rhazimanine from A. scholaris, Phytochemistry, 1987, 26, 2139.
30. Ratnayake, Chittra K., Lakshmi, S.R., Arambewela, DeSilva, K.T., Atta−ur−Rahman, and Alvi, K.A., Alkaloid of Alstonia macrophylla, Phytochemistry, 1987, 26, 868.
31. Ahmad, V.U. and Alvi, K.A., Deacetoxybrachycarpone, Carbralealactone and Ursolic Acid from Cleome. brachycarpa, Phytochemistry, 1987, 26, 315.
32. Atta−ur−Rahman, Silva, W.S.J., Alvi, K.A., DeSilva, K.T., Nb−Demethylalstophylline Oxindole, and Oxindole Alkaloid from Alstonia macrophylla, Phytochemistry, 1987, 26, 856−868.
33. Atta−ur−Rahman, Silva, W.S.J., Alvi, K.A., DeSilva, K.T., Isolation and Structural Studies on the Alkaloids of Petechia ceylanica, Phytochemistry, 1987, 26, 543.
34. Atta−ur−Rahman, Alvi, K.A., Abbas, S.A., and Voelter, W., 19,20−Z−Vallesamine and 19,20−E−Vallesamine from A. scholaris, Heterocyclic, 1987, 26, 413.
35. De Silva, L.B., Herath, W.H.M.W., Navaratne, K.M., Ahmad, V.U. and Alvi, K.A., An iridoid glycoside from Wendlandia bicuspidata, J. Nat. Prod., 1987, 50, 1184.
36. Ahmad, V.U., Alvi, K.A., and Khan, M.A., The Molecular Structure and Absolute Configuration of Brachycarpone, a new trinortriterpenoid Dilactone from Cleome brachycarpa, J. Nat. Prod., 1986, 49, 249.
37. Ahmad, V.U. and Alvi, K.A., Deacetoxybrachycarpone, a trinorterpenoid from Cleome brachycarpa, Phytochemistry, 1986, 26, 315.
38. Atta−ur−Rahman, Alvi, K.A., and Muzaffar, A., Isolation and 1H and 13C−NMR Studies on 19,20−dihydrocondylocarpine. An Alkaloid from the Leaves of Alstonia. scholaris and Ervatamia caronaria, Planta medica, 1986, 4, 325.
39. Atta−ur−Rahman, Alvi, K.A., Fast−Atomic−Bombardment Mass Spectrometry Using Complexes Containing Potassium hexacyanoferrate (II) and other metal Salts, Org. Mass Spectrom, 1986, 21, 163.
40. Atta−ur−Rahman, Asif, M., Firdous, S., Fatima, J., and Alvi, K.A., 1H and 13C−NMR Studies on Picralstonie from Alstonia. scholaris, J. Chem. Soc. Pak., 1986, 8, 421.
41. Atta−ur−Rahman, Asif, M., Ghazala, M., Fatima, J., and Alvi, K.A., Scholaricine, an Alkaloid from Alstonia. scholaris, Phytochemistry, 1985, 24, 2771.
